Conchito

A smartphone-read ultrasensitive and quantitative saliva test for COVID-19 Abstract Improved point-of-care COVID-19 assays are urgently needed to improve disease control efforts, but current assays lack the sensitivity of the RT-PCR gold-standard. Herein we describe the development of a portable, ultrasensitive saliva-based COVID-19 assay with a 15 minute sample-to-answer time that does not require RNA isolation or laboratory equipment. This assay uses CRISPR/Cas12a activity to enhance signal from an amplified viral RNA target, which is stimulated by laser diode integrated into a smartphone-based fluorescence microscope readout device. This device robustly quantified viral load over a broad linear range (1-105 copies/μL) and exhibited a limit of detection (0.38 copies/μL) below that of the RT-PCR reference assay. SARS-CoV-2 RNA levels read by CRISPR were similar in patient saliva versus nasal swab samples and viral loads measured by RT-PCR and the smartphone-read CRISPR assay demonstrated good correlation, supporting the potential utility of this portable assay device for saliva-based point-of-care COVID-19 diagnosis.

Alergija je verovatno na PEG ... polietilen glikol odnosno polyethylene glycol.

Laboratorij nivo BSL-3 BSL-4

Postoje više vrsta tzv. biološke opasnosti odnonso biološke zaštite... BSL... Biosafety Level PPE...personal protective equipment. Po pravilima sterilnog rada trebaju imati posebnu sobu gde se preobuku u PPE. Kad se preoblače, treba neko da ih nagleda, kako se oblače. Posotoji poseban red kako šta obuči. Posle se ulazi u poseban prostor, gde se vrši sterilizacija pravilno obučene osobe. Sledi posle ulazak u prostora gde se radi sa mikrobom. Taj prostor morai imati posebnu ventilaciju, zatovrene prozore, itd. Kad se završi sa radom sledi izlazak, sterilizacija zaposlenog, opet presvlačenje (u pravom vrstnom redu). Treba posle tu PPE odneti negde (da nema kakve kontaaminacije). Ovaj znak je vrlo popularan: = biohazard Na kraju sve zavisi od toga koliko imaš para.. više para, bolja oprema, bolji kadar, više kadra -> kadar je manje umoran, manje če praviti grešaka, manje če biti zaraženih među zaposlenima, rad je kvalitetniji, itd.